In-vitro Maturation Of Human Oocytes: The Mcgill Protocols (The Second Edition)

In-Vitro Maturation of Human Oocytes: The McGill Protocols, by Seang Lin Tan, Suresh Nair,and Michael Dahan, is a comprehensive guide to the clinical application of IVM in assistedreproductive technologies. Drs. Tan, Nair, and Dahan—three outstanding pioneers in thisfield—detail how IVM is performed at McGill University’s Reproductive Centre, one of theleading IVM centers in the world.Long before the clinical use of in vitro fertilization was established, in vitro maturation wasconducted in 1935 in rabbit oocytes. The first successful human IVM cycles were reported in theearly 1990s. Tan, Nair, and Dahan present an elegant overview of IVM’s early history throughits systematic development and contemporary utility. This superb manual describes IVMstimulation and triggering protocols, in addition to methods of oocyte collection, culture, andassessment. They describe a new pseudo-double lumen “Steiner-Tan” egg collection needle,which has been suggested to produce better results than regular IVM needles. The role of culturemedia in supporting optimal oocyte development and IVM in combination with natural-cycleIVF is highlighted. Readers are also introduced to capacitation IVM or CAPA IVM, a two-stepalternative procedure that several studies have suggested produces higher maturation rates thanstandard IVM without an hCG trigger. Read more